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Extrusion-Based 3D Bioprinting of Gradients of Stiffness, Cell Density, and Immobilized Peptide Using Thermogelling Hydrogels.

Merve KuzucuGrace VeraMarco BeaumontSascha FischerPan WeiV Prasad ShastriAurelien Forget
Published in: ACS biomaterials science & engineering (2021)
To study biological processes in vitro, biomaterials-based engineering solutions to reproduce the gradients observed in tissues are necessary. We present a platform for the 3D bioprinting of functionally graded biomaterials based on carboxylated agarose, a bioink amendable by extrusion bioprinting. Using this bioink, objects with a gradient of stiffness and gradient of cell concentration were printed. Functionalization of carboxylated agarose with maleimide moieties that react in minutes with a cysteine-terminated cell-adhesion peptide allowed us to print objects with a gradient of an immobilized peptide. This approach paves the way toward the development of tissue mimics with gradients.
Keyphrases
  • cell adhesion
  • single cell
  • cell therapy
  • tissue engineering
  • drug delivery
  • magnetic nanoparticles
  • hyaluronic acid
  • mesenchymal stem cells
  • drug release