LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet.
Eiji UsukuraYuhki YanaseAyumi IshijimaThasaneeya KubokiSatoru KidoakiKoichi OkamotoKaoru TamadaPublished in: PloS one (2017)
This paper reports our original technique for visualizing cell-attached nanointerfaces with extremely high axial resolution using homogeneously excited localized surface plasmon resonance (LSPR) on self-assembled silver nanoparticle sheets. The LSPR sheet can confine and enhance the fluorescence at the nanointerface, which provides high signal-to-noise ratio images of focal adhesion at the cell-attached interface. The advantage of this LSPR-assisted technique is its usability, which provides comparable or higher-quality nanointerfacial images than TIRF microscopy, even under epifluorescence microscopy. We also report the cytotoxicity of silver nanoparticles, as determined via morphological analysis of adherent cells on the sheet.
Keyphrases
- single molecule
- fluorescence imaging
- gold nanoparticles
- optical coherence tomography
- single cell
- deep learning
- high resolution
- silver nanoparticles
- cell therapy
- induced apoptosis
- convolutional neural network
- high speed
- photodynamic therapy
- healthcare
- living cells
- label free
- staphylococcus aureus
- health information
- endoplasmic reticulum stress
- cell cycle arrest
- cell death
- biofilm formation
- adverse drug
- iron oxide
- electron transfer