Quantification of N6-formylated lysine in bacterial protein digests using liquid chromatography/tandem mass spectrometry despite spontaneous formation and matrix effects.

Jacob S FolzJenelle A PattersonAndrew D HansonOliver Fiehn

Published in: Rapid communications in mass spectrometry : RCM (2021)

Spontaneous formation of N6-formyl lysine, N6-acetyl lysine, N2-formyl lysine, and N2-acetyl lysine needs to be subtracted from biologically formed lysine modifications when quantifying these epimetabolites in biological samples.

Keyphrases

liquid chromatography tandem mass spectrometry
amino acid
simultaneous determination
ms ms
mass spectrometry
small molecule
solid phase extraction
protein protein
liquid chromatography