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Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper.

Angèle H M BénardEtienne GuenouMaria FookesJerome AteudjieuWatipaso KasambaraMatthew SieverStanislas RebaudetJacques BoncyPaul AdrienRenaud PiarrouxDavid A SackNicholas ThomsonAmanda K Debes
Published in: PLoS neglected tropical diseases (2019)
WGS results allowed for mapping of short reads from APW-enriched specimen and culture isolate spotted filter papers. This provided valuable molecular epidemiological sequence information on V. cholerae strains from remote, low-resource settings. These results identified the presence of co-infecting pathogens while providing rare insight into the specific V. cholerae strains causing outbreaks in cholera-endemic areas.
Keyphrases
  • escherichia coli
  • high resolution
  • gram negative
  • high density
  • antimicrobial resistance
  • healthcare
  • multidrug resistant
  • amino acid