A Fluoran-Based Cu2+-Selective Fluorescent Probe and its Application in Cell Imaging.
Qi QiuBo YuKun HuangDabin QinPublished in: Journal of fluorescence (2020)
Based on a fluoran skeleton, we herein reported a new fluorescent probe FLACu for Cu2+ by facile modification of the spirolactone of dye 1 with phenylhydrazine. Taking advantage of the spirolactam moiety, FLACu only delivers a specific "off-on" fluorescence signal output towards Cu2+ when treated with various metal ions, which has been well elucidated as the result of spirolactam ring opening and hydrolysis processes induced by copper ion. Meanwhile, the limit of detection (LOD) for FLACu has been estimated to be 35.4 nM and a pH range of 5.0-9.0 is evaluated to be suitable for Cu2+ detection. Significantly, FLACu shows low cell cytotoxicity and can be further ultilzied in the detection of exogenous Cu2+ in the living Hela cells.
Keyphrases
- fluorescent probe
- aqueous solution
- living cells
- metal organic framework
- loop mediated isothermal amplification
- real time pcr
- single cell
- label free
- induced apoptosis
- quantum dots
- high resolution
- cell cycle arrest
- single molecule
- stem cells
- bone marrow
- photodynamic therapy
- cell proliferation
- newly diagnosed
- mesenchymal stem cells
- sensitive detection
- signaling pathway