Forming megakaryocytes from murine-induced pluripotent stem cells by the inducible overexpression of supporting factors.
Katharina CullmannMagdalena JahnMarkus SpindlerFranziska SchenkGeorgi ManukjanAdele MucciDoris SteinemannKlaus BollerHarald SchulzeMarkus BenderThomas MoritzUte ModlichPublished in: Research and practice in thrombosis and haemostasis (2020)
We demonstrate that the use of rtTA-M2 transgenic iPSCs transduced with Tet-inducible retroviral vectors allowed for gene expression at later time points during differentiation. With this strategy we could identify factors that increased in vitro MK production.