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Self-assembly of molecular beacons through metal ion coordination for fluorescence imaging of miRNA in living cells.

Chan YangFenfen LiLiuting MoWeiying Lin
Published in: Luminescence : the journal of biological and chemical luminescence (2023)
Fluorescence nanosensors based on functional nucleic acids have been explored as a powerful sensing platform for disease-relevant miRNAs. This work developed a new hybrid nanosensor (Zr-B) through coordination-driven self-assembly of Zr ions and beacons. The prepared nanosensor exhibited high loading efficiency of beacons and could achieve sensitive and specific detection for miRNAs. The hybrid nanosensor could transfer beacons into living cells efficiently and maintain high stability and biocompatibility in the biological environment, achieving effective miRNA fluorescence imaging in living cells. Therefore, the resultant nanosensor holds potential for applications in disease diagnostics.
Keyphrases
  • living cells
  • fluorescence imaging
  • single molecule
  • fluorescent probe
  • photodynamic therapy
  • pet imaging
  • quantum dots
  • human health
  • computed tomography
  • label free
  • single cell
  • aqueous solution