Fe(III)-complex mediated bacterial cell surface immobilization of eGFP and enzymes.

Lilin FengLiang GaoDaniel F SauerYu JiHaiyang CuiUlrich Schwaneberg

Published in: Chemical communications (Cambridge, England) (2021)

We report a facile and reversible method to immobilize a broad range of His6-tagged proteins on the E. coli cell surface through Fe(iii)-metal complexes. A His6-tagged eGFP and four His6-tagged enzymes were successfully immobilized on the cell surface. Additionally, a hydrogel sheath around E. coli cells was generated by immobilized His6-tagged HRP.

Keyphrases

cell surface
escherichia coli
induced apoptosis
metal organic framework
ionic liquid
cell cycle arrest
drug delivery
magnetic nanoparticles
quantum dots
signaling pathway
cell death
endoplasmic reticulum stress
mass spectrometry
hyaluronic acid
pi k akt
reduced graphene oxide
tissue engineering