Nanolipoidal α-terpineol modulates quorum sensing regulated virulence and biofilm formation in Pseudomonas aeruginosa.
Sunil Kumar BosePradip NirbhavaneMahak BatraSanjay ChhibberKusum HarjaiPublished in: Nanomedicine (London, England) (2020)
Aim: Pseudomonas aeruginosa has emerged as a major opportunistic pathogen meaning there is an immediate need to develop efficient antivirulence agents which offer a new class of superior therapeutics. Methods: Nanostructured lipid carriers (NLCs) containing α-terpineol (αT) were developed and characterized to determine expression profiles of quorum sensing regulated genes, antivirulence activity and antibiofilm effects against P. aeruginosa. Results: The αT-NLCs had a size of 145.4 nm, polydispersity index of 0.242 and ζ-potential of -31.4 mV. They exhibited pronounced effects on the inhibition of quorum sensing mediated virulence and biofilm formation which were confirmed by molecular docking analysis and gene expression profiles. Conclusion: αT-NLCs show promise as effective antivirulence agents against P. aeruginosa in the postantibiotic era.
Keyphrases
- biofilm formation
- pseudomonas aeruginosa
- molecular docking
- candida albicans
- cystic fibrosis
- transcription factor
- genome wide
- genome wide identification
- molecular dynamics simulations
- staphylococcus aureus
- acinetobacter baumannii
- escherichia coli
- photodynamic therapy
- copy number
- small molecule
- big data
- dna methylation
- drug resistant
- multidrug resistant
- antimicrobial resistance