Fluorogenic probes for live-cell imaging of the cytoskeleton.

Gražvydas LukinavičiusLuc ReymondElisa D'EsteAnastasiya MasharinaFabian GöttfertHaisen TaAngelika GütherMathias FournierStefano RizzoHerbert WaldmannClaudia BlaukopfChristoph SommerDaniel W GerlichHans-Dieter ArndtStefan W HellKai Johnsson

Published in: Nature methods (2014)

We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.

Keyphrases

living cells
single molecule
fluorescence imaging
high resolution
fluorescent probe
photodynamic therapy
oxidative stress
mass spectrometry
single cell
cell migration
high throughput
small molecule
spinal cord injury
high speed
label free