Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast.
Bingyin PengManuel R PlanAlexander CarpenterLars K NielsenClaudia E VickersPublished in: Biotechnology for biofuels (2017)
Direct comparison of different transcriptional regulatory mechanisms clearly demonstrated that coupling the output strength to the fermentation stage is important to optimize the growth fitness and overall productivities of engineered cells in industrially relevant processes. Applying different well-characterized promoters with the same induction behaviour mitigates against the risks of homologous sequence-mediated genetic instability. Using these approaches, we significantly improved sesquiterpene production in yeast.
Keyphrases
- saccharomyces cerevisiae
- induced apoptosis
- transcription factor
- room temperature
- cell cycle arrest
- physical activity
- body composition
- dna damage
- cell wall
- dna repair
- genome wide
- human health
- oxidative stress
- cell proliferation
- signaling pathway
- radiation therapy
- climate change
- network analysis
- electron transfer
- amino acid