Cell Cycle Modulation through Physical Confinement in Micrometer-Thick Hydrogel Sheaths.
Wildan MubarokKelum Chamara Manoj Lakmal ElvitigalaHiroto NakayaTomoki HottaShinji SakaiPublished in: Langmuir : the ACS journal of surfaces and colloids (2024)
Recently, surface engineering of the cell membrane with biomaterials has attracted great attention for various biomedical applications. In this study, we investigated the possibility of modulating cell cycle progression using alginate and gelatin-based hydrogel sheaths with a thickness of ∼1 μm. The hydrogel sheath was formed on cell surfaces through cross-linking catalyzed by horseradish peroxidase immobilized on the cell surface. The hydrogel sheath did not decrease the viability (>95% during 2 days of culture) of the human cervical carcinoma cell line (HeLa) expressing the fluorescent ubiquitination-based cell cycle indicator 2 (HeLa/Fucci2). Coating the HeLa/Fucci2 cells with the hydrogel sheath resulted in a cell cycle arrest in the G2/M phase, which can be caused by the reduced F-actin formation. As a result of this cell cycle arrest, an inhibition of cell growth was observed in the HeLa/Fucci2 cells. Taken together, our results demonstrate that the hydrogel sheath coating on the cell surface is a feasible approach to modulating cell cycle progression.
Keyphrases
- cell cycle
- cell cycle arrest
- cell death
- tissue engineering
- pi k akt
- cell proliferation
- cell surface
- drug delivery
- hyaluronic acid
- wound healing
- signaling pathway
- physical activity
- single cell
- mental health
- stem cells
- induced apoptosis
- optical coherence tomography
- quantum dots
- working memory
- ionic liquid
- bone marrow
- single molecule
- staphylococcus aureus
- biofilm formation
- endoplasmic reticulum stress
- induced pluripotent stem cells
- high speed