Bovine Serum Albumin-Conjugated Red Emissive Gold Nanocluster as a Fluorescent Nanoprobe for Super-resolution Microscopy.
Aditya YadavNavneet Chandra VermaChethana RaoPushpendra M MishraAmit JaiswalChayan Kanti NandiPublished in: The journal of physical chemistry letters (2020)
The gold nanocluster (GNC), because of its interesting photoluminescence properties and easy renal clearance from the body, has tremendous biomedical applications. Unfortunately, it has never been explored for super-resolution microscopy (SRM). Here, we present a protein-conjugated red emissive GNC for super-resolution radial fluctuation (SRRF) of the lysosome in HeLa cells. The diameter of the lysosome obtained in SRRF is ∼59 nm, which is very close to the original diameter of the smallest lysosome in HeLa cells. Conjugation of protein to GNC aided in the specific labeling of the lysosome. We hope that GNC not only will replace some of the common dyes used in SRM but due to its electron beam contrast could also be used as a multimodal probe for several other correlative bioimaging techniques.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- cell cycle arrest
- induced apoptosis
- quantum dots
- photodynamic therapy
- cell death
- high resolution
- pi k akt
- signaling pathway
- endoplasmic reticulum stress
- high speed
- optical coherence tomography
- protein protein
- label free
- pain management
- light emitting
- small molecule
- ultrasound guided
- silver nanoparticles
- chronic pain
- mass spectrometry