Redox-State-Mediated Regulation of Cytochrome c Release in Apoptosis Revealed by Surface-Enhanced Raman Scattering on Nickel Substrates.
Jinyu ZhuMuwei JiangHao MaHaijing ZhangWeina ChengJunbo LiLinjun CaiXiao-Xia HanBing ZhaoPublished in: Angewandte Chemie (International ed. in English) (2019)
The interaction of cytochrome c (Cyt c) with cardiolipin (CL) is believed to play an important role in the initial events of apoptosis. Herein, we investigate the structural changes of CL-bound Fe2+ Cyt c and the correlation with Cyt c release through surface-enhanced Raman spectroscopy (SERS) on nickel substrates. The SERS results together with molecular dynamics simulation reveal that Fe2+ Cyt c undergoes autoxidation and a relatively larger conformational alteration after binding with CL, inducing higher peroxidase activity of Cyt c and higher permeability of the CL membrane compared with those induced by the Fe3+ Cyt c. The proapoptotic activity and SERS effect of the Ni nanostructures allow the in situ study of the redox-state-dependent Cyt c release from isolated mitochondria, which reveals for the first time that the ferrous state of Cyt c most likely plays a more important role in triggering apoptosis.
Keyphrases
- raman spectroscopy
- molecular dynamics simulations
- oxidative stress
- cell death
- gold nanoparticles
- endoplasmic reticulum stress
- metal organic framework
- cell cycle arrest
- sensitive detection
- molecular docking
- endothelial cells
- single cell
- genome wide
- hydrogen peroxide
- signaling pathway
- label free
- pi k akt
- aqueous solution
- visible light