Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis.

Liuyang HuBing HanQin TongHui XiaoDonglin Cao

Published in: The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale (2020)

Multiplex real-time PCR with fluorescence MCA assay is a sensitive, specific, high-throughput, and cost-effective method to detect multiple bacterial pathogens simultaneously.

Keyphrases

real time pcr
high throughput
gram negative
single molecule
energy transfer
high resolution
antimicrobial resistance
single cell