Chemical synthesis of natural and azido-modified UDP-rhamnose and -arabinofuranose for glycan array-based characterization of plant glycosyltransferases.

Irene PasiniColin RuprechtUwe OsswaldAndreas BittmannLina MaltrovskyCecilia Romanò Mads Hartvig Clausen Fabian Pfrengle

Published in: Chemical communications (Cambridge, England) (2024)

Chemical syntheses of UDP-rhamnose and UDP-arabinofuranose and respective azido-modified analogues are reported. The prepared substrates are useful for the glycan array-based analysis of glycosyltransferases, as exemplified with the plant cell wall-biosynthetic enzymes Pv XAT3, At RRT4 and Pt RRT5.

Keyphrases

cell wall
high throughput
high resolution
cell surface
high density
molecular docking
mass spectrometry