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DNA Nanolithography Enables a Highly Ordered Recognition Interface in a Microfluidic Chip for the Efficient Capture and Release of Circulating Tumor Cells.

Jialu ZhangBingqian LinLingling WuMengjiao HuangXingrui LiHuimin ZhangJia SongWei WangGang ZhaoYanLing SongChaoyong James Yang
Published in: Angewandte Chemie (International ed. in English) (2020)
Microfluidic chips with nano-scale structures have shown great potential, but the fabrication and cost issues restrict their application. Herein, we propose a conceptually new "DNA nanolithography in a microfluidic chip" by using sub-10 nm three-dimensional DNA structures (TDNs) as frameworks with a pendant aptamer at the top vertex (ApTDN-Chip). The nano-scale framework ensures that the aptamer is in a highly ordered upright orientation, avoiding the undesired orientation or crowding effects caused by conventional microfluidic interface fabrication processes. Compared with a monovalent aptamer modified chip, the capture efficiency of ApTDN-Chip was enhanced nearly 60 % due to the highly precise dimension and rigid framework of TDNs. In addition, the scaffolds make DNase I more accessible to the aptamer with up to 83 % release efficiency and 91 % cell viability, which is fully compatible with downstream molecular analysis. Overall, this strategy provides a novel perspective on engineering nano-scaffolds to achieve a more ordered nano-topography of microfluidic chips.
Keyphrases
  • circulating tumor cells
  • circulating tumor
  • gold nanoparticles
  • sensitive detection
  • tissue engineering
  • label free
  • high throughput
  • cell free
  • single molecule
  • photodynamic therapy
  • low cost