The field of synthetic glycobiotechnology encompasses the synthesis and modification of free carbohydrates and carbohydrates linked to biomolecules. Our group develops bio-catalytic processes for the synthesis of carbohydrate building blocks, so-called sugar nucleotides, and cell-free multi-enzyme cascades to tailor carbohydrates linked to proteins. The technology can eventually help to advance our understanding of the roles of specific carbohydrates in nutrition and medicine and contribute to human health and well-being.