G-CSF-induced macrophage polarization and mobilization may prevent acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation.
Qi WenYuan KongHong-Yan ZhaoYuan-Yuan ZhangTing-Ting HanYu WangLan-Ping XuXiao-Hui ZhangXiao-Jun HuangPublished in: Bone marrow transplantation (2019)
Macrophages (MΦs) are an important immune cell population that are essential for tissue homeostasis and disease pathogenesis. MΦs are now classified as either M1, which produce pro-inflammatory cytokines, or M2, which produce antiinflammatory cytokines. The impact of granulocyte colony-stimulating factor (G-CSF) on MΦs in humans is unclear. Moreover, little is known about the association between MΦ subsets in allografts and the occurrence of acute graft-versus-host disease (aGVHD) in patients who undergo allogeneic hematopoietic stem cell transplantation (allo-HSCT). In the current study, we found that the M1/M2 ratio was markedly decreased in both G-CSF-treated bone marrow (post-BM) and G-CSF-treated peripheral blood from healthy donors. Post-BM MΦs exhibited reduced migration and increased phagocytosis. Moreover, post-BM MΦs reduced the percentage of Th1 and Tc1 lineages and increased the percentage of Th2, Tc2, and Treg lineages. Patients who received BM grafts with a higher M1/M2 ratio exhibited a higher incidence of grade 2-4 aGVHD. In summary, our data indicate that G-CSF decreases the M1/M2 ratio in BM grafts from healthy donors, which may contribute to preventing the occurrence of grade 2-4 aGVHD in patients after allo-HSCT.
Keyphrases
- allogeneic hematopoietic stem cell transplantation
- peripheral blood
- acute myeloid leukemia
- acute lymphoblastic leukemia
- bone marrow
- liver failure
- newly diagnosed
- drug induced
- end stage renal disease
- risk assessment
- respiratory failure
- ejection fraction
- cerebrospinal fluid
- chronic kidney disease
- mesenchymal stem cells
- risk factors
- hematopoietic stem cell
- big data
- artificial intelligence
- high glucose
- intensive care unit
- acute respiratory distress syndrome
- patient reported