Characterization of the Glycosyltransferase and Methyltransferase Encoded Remotely from the Actinopyrone Biosynthetic Gene Cluster Enables Access to Diverse Analogues.
Huaran ZhangNaying GongHua ZhangQinglian LiJunying MaXiao-Yi WeiWen-Li LiJianhua JuPublished in: Organic letters (2022)
The actinopyrone biosynthetic gene cluster ( atpn ) lacks glycosyl- and methyltransferase genes, yet its product clearly calls for such enzymes. Using bioinformatics and biochemical methods, we confirmed that the mt3913 and gt723 genes, well beyond the atpn cluster boundaries, encode methyltransferase and glycosyltransferase, respectively. Moreover, homologous protein GT1507 enabled us to produce 14 non-natural actinopyrone analogues. PM050463 ( 3 ) was found to display potent anti- Helicobacter pylori activity and no signs of cytotoxicity.
Keyphrases
- helicobacter pylori
- genome wide identification
- genome wide
- genome wide analysis
- helicobacter pylori infection
- copy number
- transcription factor
- molecular docking
- dna damage
- particulate matter
- air pollution
- bioinformatics analysis
- dna repair
- risk assessment
- amino acid
- structure activity relationship
- small molecule
- gene expression
- binding protein
- oxidative stress
- water soluble