Recent advances in soluble decellularized extracellular matrix for heart tissue engineering and organ modeling.
Golara KafiliHannaneh KabirAmirhossein Jalali KandeloosElham GolafshanSara GhasemiShohreh MashayekhanNayere TaebniaPublished in: Journal of biomaterials applications (2023)
Despite the advent of tissue engineering (TE) for the remodeling, restoring, and replacing damaged cardiovascular tissues, the progress is hindered by the optimal mechanical and chemical properties required to induce cardiac tissue-specific cellular behaviors including migration, adhesion, proliferation, and differentiation. Cardiac extracellular matrix (ECM) consists of numerous structural and functional molecules and tissue-specific cells, therefore it plays an important role in stimulating cell proliferation and differentiation, guiding cell migration, and activating regulatory signaling pathways. With the improvement and modification of cell removal methods, decellularized ECM (dECM) preserves biochemical complexity, and bio-inductive properties of the native matrix and improves the process of generating functional tissue. In this review, we first provide an overview of the latest advancements in the utilization of dECM in in vitro model systems for disease and tissue modeling, as well as drug screening. Then, we explore the role of dECM-based biomaterials in cardiovascular regenerative medicine (RM), including both invasive and non-invasive methods. In the next step, we elucidate the engineering and material considerations in the preparation of dECM-based biomaterials, namely various decellularization techniques, dECM sources, modulation, characterizations, and fabrication approaches. Finally, we discuss the limitations and future directions in fabrication of dECM-based biomaterials for cardiovascular modeling, RM, and clinical translation.
Keyphrases
- tissue engineering
- extracellular matrix
- cell migration
- signaling pathway
- induced apoptosis
- cell proliferation
- left ventricular
- gene expression
- heart failure
- single cell
- drinking water
- emergency department
- transcription factor
- pseudomonas aeruginosa
- cell cycle arrest
- epithelial mesenchymal transition
- mass spectrometry
- cell cycle
- endoplasmic reticulum stress
- oxidative stress
- stem cells
- mesenchymal stem cells
- electronic health record
- biofilm formation