A Short Peptide Designed from Late Embryogenesis Abundant Protein Enhances Acid Tolerance in Escherichia coli.
Khaled MetwallyShinya IkenoPublished in: Applied biochemistry and biotechnology (2020)
Unsuitable pH is a major limiting factor for all organisms, and a low pH can lead to organism death. Late embryogenesis abundant (LEA) peptides confer tolerance to abiotic stresses including salinity, drought, high and low temperature, and ultraviolet radiation same as the LEA proteins from which they originate. In this study, LEA peptides derived from group 3 LEA proteins of Polypedilum vanderplanki were used to enhance low pH tolerance. Recombinant Escherichia coli BL21 (DE3) cells expressing the five designed LEA peptides were grown at pH 4, 3, and 2. The transformants showed higher growth capacity at low pH as compared to control cells. These results indicate that LEA peptide could prevent E. coli cell death under low pH conditions.
Keyphrases
- escherichia coli
- cell death
- cell cycle arrest
- induced apoptosis
- oxidative stress
- climate change
- radiation therapy
- staphylococcus aureus
- cystic fibrosis
- multidrug resistant
- radiation induced
- pseudomonas aeruginosa
- signaling pathway
- klebsiella pneumoniae
- pi k akt
- endoplasmic reticulum stress
- genome wide identification
- cell free
- transcription factor