Visual Stimulus Detection Correlates with the Consistency of Temporal Sequences within Stereotyped Events of V1 Neuronal Population Activity.

Temporally coactive neurons have been hypothesized to form functional assemblies that might subserve different functions in the brain, but many of these proposed functions have not yet been experimentally tested. We used two-photon calcium imaging in V1 of mice performing a stimulus detection task to study the relation of assembly activity to the behavioral detection of visual stimuli. We found that the presence of recurring assemblies per se was not correlated with behavior, and these assemblies did not appear to serve a function in the coding of stimulus orientation. Instead, we found that activity in V1 is characterized by population events of varying membership, within which the consistency of the temporal sequence of neuronal activation is correlated with stimulus detection.