Metasurfaces-driven Hyperspectral Imaging via Multiplexed Plasmonic Resonance Energy Transfer.

Obtaining single molecular level fingerprints of biomolecules and electron transfer dynamic imaging in living cells are critically demanded in postgenomic life sciences and medicine. However, the possible solution called Plasmonic Resonance Energy Transfer (PRET) spectroscopy remains challenging due to the fixed scattering spectrum of a plasmonic nanoparticle and limited multiplexing. Here, w e report multiplexed metasurfaces-driven PRET hyperspectral imaging to probe biological light-matter interactions. W e first design pixelated metasurfaces with engineered scattering spectra over the entire visible range by the precision nanoengineering of gap plasmon and grating effects of metasurface clusters. W e create pixelated metasurfaces and optically characterize their full darkfield coloration with visible color palettes and high-resolution color printings of the art pieces. Furthermore, w e apply three different biomolecules (i.e., chlorophyll a, chlorophyll b, and cytochrome c) on metasurfaces for color palettes to obtain selective molecular fingerprint imaging due to the unique biological light-matter interactions with application-specific biomedical metasurfaces. This metasurface-driven PRET hyperspectral imaging will open up a new path for multiplexed real-time molecular sensing and imaging methods. This article is protected by copyright. All rights reserved.