Overall, this study provides the first example of recovery plus characterization of B. melitensis RNA from in vivo lymph node infection, and demonstrates that the coincidence cloning technique is a useful tool for characterizing in vivo transcriptional changes in Brucella species. Genes upregulated in long-term infection in this data set, including many genes not previously demonstrated to be virulence factors in mice or macrophage experiments, are candidates of future interest for potential roles in Brucella persistence in natural host systems.
Keyphrases
- gene expression
- lymph node
- genome wide
- dna methylation
- escherichia coli
- genome wide identification
- pseudomonas aeruginosa
- staphylococcus aureus
- adipose tissue
- bioinformatics analysis
- electronic health record
- biofilm formation
- transcription factor
- current status
- squamous cell carcinoma
- neoadjuvant chemotherapy
- candida albicans
- high fat diet induced
- genome wide analysis
- skeletal muscle
- machine learning
- metabolic syndrome
- human health
- sentinel lymph node
- insulin resistance
- artificial intelligence