Determination of organic acids for predicting sourness intensity of tea beverage by liquid chromatography-tandem mass spectrometry and chemometrics methods.
Meiyan LiuLijuan ShiJie GuoYing GuSiyu LiLunzhao YiDabing RenBoyan LiPublished in: Journal of separation science (2024)
The contents of organic acids (OAs) in tea beverage and their relationship with taste intensity have not been fully understood. In this work, a rapid (10 min for a single run) and sensitive (limits of quantification: 0.0044-0.4486 µg/mL) method was developed and validated for the simultaneous determination of 17 OAs in four types of tea, based on liquid chromatography-tandem mass spectrometry with multiple reaction monitoring mode. The contents of 17 OAs in 96 tea samples were measured at levels between 0.01 and 11.80 g/kg (dried weight). Quinic acid, citric acid, and malic acid were determined as the major OAs in green, black, and raw pu-erh teas, while oxalic acid and tartaric acid exhibited the highest contents in ripe pu-erh tea. Taking the OAs composition as input features, a partial least squares regression model was proposed to predict the sourness intensity of tea beverages. The model achieved a root-mean-square error of 0.58 and a coefficient of determination of 0.84 for the testing set. The proposed model provides a theoretical way to evaluate the sensory quality of tea infusion based on its chemical composition.
Keyphrases
- liquid chromatography tandem mass spectrometry
- simultaneous determination
- solid phase extraction
- high performance liquid chromatography
- ms ms
- tandem mass spectrometry
- molecularly imprinted
- gas chromatography mass spectrometry
- high intensity
- body mass index
- low dose
- computed tomography
- magnetic resonance
- mass spectrometry
- quality improvement
- sensitive detection