Induction of the PERK-eIF2α-ATF4 Pathway in M1 Macrophages under Endoplasmic Reticulum Stress.

O E Kolodeeva O E Kolodeeva D A Averinskaya Julia Makarova

Published in: Doklady. Biochemistry and biophysics (2024)

Translation inhibition can activate two cell death pathways. The first pathway is activated by translational aberrations, the second by endoplasmic reticulum (ER) stress. In this work, the effect of ribosome-inactivating protein type II (RIP-II) viscumin on M1 macrophages derived from the THP-1 cell line was investigated. The number of modified ribosomes was evaluated by real-time PCR. Transcriptome analysis revealed that viscumin induces the ER stress activated by the PERK sensor.

Keyphrases

endoplasmic reticulum stress
endoplasmic reticulum
induced apoptosis
real time pcr
cell death
copy number
protein protein
dna methylation
atomic force microscopy
small molecule
cell cycle arrest
cell proliferation
signaling pathway
genome wide
pi k akt