Metabolites with Anti-Inflammatory Activity from the Mangrove Endophytic Fungus Diaporthe sp. QYM12.
Yan ChenGe ZouWencong YangYingying ZhaoQi TanLin ChenJinmei WangChangyang MaWenyi KangZhi-Gang ShePublished in: Marine drugs (2021)
One new diterpenoid, diaporpenoid A (1), two new sesquiterpenoids, diaporpenoids B-C (2,3) and three new α-pyrone derivatives, diaporpyrones A-C (4-6) were isolated from an MeOH extract obtained from cultures of the mangrove endophytic fungus Diaporthe sp. QYM12. Their structures were elucidated by extensive analysis of spectroscopic data. The absolute configurations were determined by electronic circular dichroism (ECD) calculations and a comparison of the specific rotation. Compound 1 had an unusual 5/10/5-fused tricyclic ring system. Compounds 1 and 4 showed potent anti-inflammatory activities by inhibiting the production of nitric oxide (NO) in lipopolysaccharide (LPS)-induced RAW264.7 cells with IC50 values of 21.5 and 12.5 μM, respectively.
Keyphrases
- lps induced
- anti inflammatory
- inflammatory response
- nitric oxide
- induced apoptosis
- signaling pathway
- cell cycle arrest
- molecular docking
- molecular dynamics
- molecular dynamics simulations
- density functional theory
- high resolution
- electronic health record
- hydrogen peroxide
- toll like receptor
- big data
- endoplasmic reticulum stress
- cell death
- machine learning
- artificial intelligence
- deep learning
- cell proliferation
- mass spectrometry
- structure activity relationship