Assembly of branched ubiquitin oligomers by click chemistry.

Xiaohui ZhaoMartin Scheffner Andreas Marx

Published in: Chemical communications (Cambridge, England) (2019)

Ubiquitin monomers functionalized with an azide or multiple alkynes were utilized for the assembly of branched ubiquitin oligomers (K6/K11, K11/K48, K11/K63, K6/K11/K48) by click chemistry. The oligomers resist deubiquitylase-catalysed hydrolysis and exhibit stability in eukaryotic cell lysates.

Keyphrases

small molecule
single cell
drug discovery
cell therapy
stem cells
mesenchymal stem cells
mass spectrometry