Development of a multiplex droplet digital PCR method for detection and monitoring of Mycobacterium tuberculosis and drug-resistant tuberculosis.

Yu Jeong Choi Yoonjung Kim M D Hye Jung Park Dokyun Kim Hyuk Min Lee Young Ah Kim Kyung-A Lee

Published in: Annals of clinical microbiology and antimicrobials (2024)

We developed a sensitive and accurate multiplex ddPCR assay that can detect the presence of tuberculosis quantitatively and resistance-conveying mutations concurrently. This tool could aid clinicians in the diagnosis and treatment monitoring of tuberculosis.

Keyphrases

mycobacterium tuberculosis
drug resistant
real time pcr
high throughput
pulmonary tuberculosis
multidrug resistant
acinetobacter baumannii
hiv aids
single cell
palliative care
loop mediated isothermal amplification
hiv infected
human immunodeficiency virus
label free
quantum dots