Marine Natural Compound (Neviotin A) Displays Anticancer Efficacy by Triggering Transcriptomic Alterations and Cell Death in MCF-7 Cells.
Quaiser SaquibStefan SchwaigerMostafa AlilouSarfaraz AhmedMaqsood A SiddiquiJaved AhmadMohammad FaisalEslam M Abdel-SalamRizwan WahabAdnan J Al-RehailyHermann StuppnerAbdulaziz A Al-KhedhairyPublished in: Molecules (Basel, Switzerland) (2023)
We investigated the anticancer mechanism of a chloroform extract of marine sponge ( Haliclona fascigera ) (sample C) in human breast adenocarcinoma (MCF-7) cells. Viability analysis using MTT and neutral red uptake (NRU) assays showed that sample C exposure decreased the proliferation of cells. Flow cytometric data exhibited reactive oxygen species (ROS), nitric oxide (NO), dysfunction of mitochondrial potential, and apoptosis in sample C-treated MCF-7 cells. A qPCR array of sample C-treated MCF-7 cells showed crosstalk between different pathways of apoptosis, especially BIRC5 , BCL2L2 , and TNFRSF1A genes. Immunofluorescence analysis affirmed the localization of p53, bax, bcl2, MAPKPK2, PARP-1, and caspase-3 proteins in exposed cells. Bioassay-guided fractionation of sample C revealed Neviotin A as the most active compound triggering maximum cell death in MCF-7, indicating its pharmacological potency for the development of a drug for the treatment of human breast cancer.
Keyphrases
- cell cycle arrest
- cell death
- induced apoptosis
- oxidative stress
- endoplasmic reticulum stress
- nitric oxide
- pi k akt
- signaling pathway
- reactive oxygen species
- endothelial cells
- gene expression
- breast cancer cells
- emergency department
- dna methylation
- young adults
- radiation therapy
- rectal cancer
- high throughput
- hydrogen peroxide
- big data