The role of caveolin-1 and endothelial nitric oxide synthase polymorphisms in susceptibility to prostate cancer.
Mahdieh AliyariDaniel Elieh-Ali-KomiAmir KianiMahmoudreza MoradiMaryam TanhapourZohreh RahimiHadi MozafariEhsan Mohammadi-NooriTayebeh PourmotabbedAsad Vaisi-RayganiFariborz BahrehmandPublished in: International journal of experimental pathology (2021)
Caveolin-1(cav-1) is overexpressed in prostate cancer (PC) and is associated with progression of the disease. We investigated the effects of CAV1-T29107A and endothelial nitric oxide synthase (eNOS) G894T polymorphisms on the serum levels of testosterone, NO and prostate-specific antigen (PSA) in patients with PC. We genotyped cav-1 and eNOS genes in 112 PC patients and 150 healthy controls by PCR-RFLP. Serum levels of NO 2 - and NO 3 - were measured using spectrophotometry, and serum levels of testosterone and PSA were measured by ELISA. The frequencies of CAV1 genotypes A/T vs. A/A according to the dominant model AT + TT vs. AA genotype and T allele were significantly higher in PC patients in comparison with the control group and considerably increased the risk of disease by 2.19-, 1.44- and 1.6-fold, respectively. AT + TT genotypes were associated significantly with the increased risk of PC in those with smoking or diabetes by 3.08-fold (P = .004). Individuals carrying concurrently the T allele of CAV1 A29107T and the T allele of eNOS G894T genes had a significantly increased risk of PC by 2.52-fold (P = .009). We did not find any significant relationship between eNOS G894T genotypes and alleles with susceptibility to PC. Our results highlighted the significance of CAV1-T29107A SNP but not (eNOS) G894T in the susceptibility to PC in our the population that we have studied.
Keyphrases
- nitric oxide synthase
- prostate cancer
- nitric oxide
- endothelial cells
- end stage renal disease
- radical prostatectomy
- newly diagnosed
- ejection fraction
- chronic kidney disease
- pi k akt
- type diabetes
- cardiovascular disease
- gene expression
- replacement therapy
- high resolution
- patient reported outcomes
- smoking cessation
- insulin resistance
- transcription factor
- glycemic control
- patient reported
- genome wide identification