Development and evaluation of a quantitative polymerase chain reaction for aquatic Streptococcus agalactiae based on the groEL gene.
W J LeighRuth N ZadoksJanina Z CostaA JaglarzKim Dawn ThompsonPublished in: Journal of applied microbiology (2020)
This rapid and sensitive qPCR assay is useful to quantify DNA copy number in the laboratory and could prove useful for detecting low levels of S. agalactiae in aquaculture systems, including Oreochromis niloticus culture.
Keyphrases
- copy number
- mitochondrial dna
- genome wide
- circulating tumor
- risk assessment
- dna methylation
- high throughput
- biofilm formation
- cell free
- single molecule
- candida albicans
- high resolution
- loop mediated isothermal amplification
- escherichia coli
- gene expression
- nucleic acid
- pseudomonas aeruginosa
- quantum dots
- circulating tumor cells
- transcription factor