A modified flow cytometry method for objective estimation of human CD4+ regulatory T cells (CD4+ Tregs) in peripheral blood, via CD4/CD25/CD45RO/FoxP3 labeling.

Asimina PetsiouStavroula A PaschouGeorgios VartholomatosKaterina ChatzigianniNikolaos KolaitisEleni GiotakiGeorge P BondinasAntonis K MoustakasAchilleas KaramoutsiosEleftheria ZervouStelios TigasAgathocles TsatsoulisGeorge K Papadopoulos

Published in: Cytometry. Part B, Clinical cytometry (2019)

Our comparison shows that CD4/CD25/CD45RO/FoxP3 panels are an objective means of estimating effector and naïve Tregs via colored dot plots, aiding thus in Treg delineation in health and detecting aberrations in disease.

Keyphrases

regulatory t cells
dendritic cells
flow cytometry
peripheral blood
healthcare
endothelial cells
nk cells
public health
mental health
immune response
social media
induced pluripotent stem cells
health promotion