Exploration of plant metabolomics variation and absorption characteristics of water-extracted Rheum tanguticum and ethanol-extracted Rheum tanguticum by UHPLC-Q-TOF-MS/MS.
Shanshan BaiDewei LuoGuoyue ZhongShilin YangHui OuyangXiaoyong RaoYulin FengPublished in: Phytochemical analysis : PCA (2023)
In this study, a combination of extraction methods, liquid chromatography-mass spectrometry (LC-MS) technology, and in vivo animal metabolism studies have been established for the screening, identification, and research of chemical active components of natural medicines. LC-MS analysis combined with plant metabolomics was used to study the differential metabolites between different extraction methods of RT. Based on UHPLC-Q-TOF-MS/MS technology, the composition and metabolism of rat plasma before and after RT administration were analysed in vivo, and 15 prototype components and 86 metabolites were detected.
Keyphrases
- mass spectrometry
- ms ms
- liquid chromatography
- high resolution mass spectrometry
- tandem mass spectrometry
- simultaneous determination
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- multiple sclerosis
- high resolution
- ultra high performance liquid chromatography
- solid phase extraction
- cell wall
- case control