Aspirin Inhibition of Prostaglandin Synthesis Impairs Mosquito Egg Development.
Duyeol ChoiMd Abdullah Al BakiShabbir AhmedYonggyun KimPublished in: Cells (2022)
Several endocrine signals mediate mosquito egg development, including 20-hydroxyecdysone (20E). This study reports on prostaglandin E 2 (PGE 2 ) as an additional, but core, mediator of oogenesis in a human disease-vectoring mosquito, Aedes albopictus . Injection of aspirin (an inhibitor of cyclooxygenase (COX)) after blood-feeding (BF) inhibited oogenesis by preventing nurse cell dumping into a growing oocyte. The inhibitory effect was rescued by PGE 2 addition. PGE 2 was found to be rich in nurse cells and follicular epithelium after BF. RNA interference (RNAi) treatments of PG biosynthetic genes, including PLA 2 and two COX-like peroxidases, prevented egg development. Interestingly, 20E treatment significantly increased the expressions of PG biosynthetic genes, while the RNAi of Shade (which is a 20E biosynthetic gene) expression prevented inducible expressions after BF. Furthermore, RNAi treatments of PGE 2 receptor genes suppressed egg production, even under PGE 2 . These results suggest that a signaling pathway of BF-20E-PGE 2 is required for early vitellogenesis in the mosquito.
Keyphrases
- aedes aegypti
- dengue virus
- zika virus
- gene expression
- signaling pathway
- genome wide
- low dose
- induced apoptosis
- primary care
- endothelial cells
- dna methylation
- genome wide identification
- bioinformatics analysis
- emergency department
- antiplatelet therapy
- stem cells
- pi k akt
- type diabetes
- cell therapy
- cell cycle arrest
- cell death
- mesenchymal stem cells
- atrial fibrillation
- genome wide analysis
- smoking cessation
- nucleic acid