Login / Signup

Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros).

Michael J CarrAkira KawaguchiMichihito SasakiGabriel GonzalezKimihito ItoYuka ThomasBernard M Hang'ombeAaron S MweeneGuoyan ZhaoDavid WangYasuko OrbaAkihiro IshiiHirofumi Sawa
Published in: Archives of virology (2016)
To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4+ T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.
Keyphrases
  • peripheral blood
  • real time pcr
  • endothelial cells
  • escherichia coli
  • copy number
  • induced pluripotent stem cells
  • nucleic acid
  • cell free
  • disease virus