PEGylated PAMAM dendrimers loading oxaliplatin with prolonged release and high payload without burst effect.
Minh Nhat HoLong Giang BachDai Hai NguyenCong Hao NguyenCuu Khoa NguyenNgoc Quyen TranNgoc Vinh NguyenThai Thanh Hoang ThiPublished in: Biopolymers (2019)
Oxaliplatin (OXA) was coupled to PEGylated polyamidoamine dendrimers of fourth generation (G4-PEG@OXA) in the comparison to PEGylated ones of odd generation (G3.5-PEG@OXA). Proton nuclear magnetic resonance and Fourier-transform infrared spectroscopy were used to confirm the successful incorporation of OXA as well as the synthesis of carrier systems. Both two types of carrier systems exhibited in sphere nanoparticle shape with size of less than 100 nm that was in the range being able to cause toxicity on cancer cells. The average drug loading efficiency (DLE) of G4-PEG@OXA was obtained at 84.63% that was higher than DLE of G3.5-PEG of 75.69%. The release kinetic of G4-PEG@OXA and G3.5-PEG@OXA did not show any burst release phenomenon while free OXA was released over 40% at the first hour. The sustainable release of OXA was achieved when it was encapsulated in these carriers, but the G4 generation liberated OXA (3.4%-6.4%) slower than G3.5 one (11.9%-22.8%). The in vitro cytotoxicities of G4-PEG@OXA were evaluated in HeLa cell lines using resazurin assay and live/dead staining test. Although the free OXA showed a rather moderate killing ability, the G4-PEG@OXA still displayed the low viability of HeLa that was better to the result of G3.5-PEG@OXA due to released OXA amount. The benefit of this system was to overcome the burst release phenomenon to minimize OXA toxicity without compromising its efficiency.
Keyphrases
- acinetobacter baumannii
- klebsiella pneumoniae
- multidrug resistant
- drug resistant
- drug delivery
- pseudomonas aeruginosa
- magnetic resonance
- escherichia coli
- blood pressure
- computed tomography
- high frequency
- oxidative stress
- high throughput
- cell death
- magnetic resonance imaging
- electronic health record
- flow cytometry
- contrast enhanced
- pi k akt