Validation of the Sw71-spheroid model with primary trophoblast cells.

Marina AlexandrovaDiana ManchorovaYuan YouAntonia TerzievaVioleta DimitrovaGil MorTanya Dimova

Published in: American journal of reproductive immunology (New York, N.Y. : 1989) (2023)

The Sw71 cell line and cultured native trophoblast cells are interchangeable regarding their EVT phenotype (HLA-C+/HLA-G+/Vim+/CK7+). The blastocyst-like spheroids sourced by both types of cells differentiate in the same time frame and function similarly. We strongly advise the use of Sw71 spheroids as blastocyst surrogate for observation on trophectoderm differentiation and function during early human implantation.

Keyphrases

induced apoptosis
cell cycle arrest
endothelial cells
cell death
oxidative stress
signaling pathway