Single-Cell Mass Spectrometry Analysis of Metabolites Facilitated by Cell Electro-Migration and Electroporation.
Zishuai LiZhengmao WangJunmin PanXiaoxiao MaWenpeng ZhangZheng OuyangPublished in: Analytical chemistry (2020)
Single-cell metabolite analysis plays an important role in biological study. While mass spectrometry is a powerful tool for identification and quantitation of metabolites, the low absolute analyte amounts in single cell and difficulty in sampling represent significant challenges in single cell analysis. In this study, we developed an effective method with a simple sampling procedure for analyzing single cells. A single cell was driven to a capillary tip through electro-migration, followed by releasing the cell contents through electroporation, into a sealed small volume (∼1.5 pL) to prevent dilution. Subsequent mass spectrometry analysis was performed directly with nanoelectrospray ionization. This method was applied for analyzing a variety of cells and monitoring the metabolic changes in response to perturbed cell culturing conditions. This method opens a new avenue for easy and rapid analysis of single cells with high sensitivity.
Keyphrases
- single cell
- rna seq
- mass spectrometry
- induced apoptosis
- high throughput
- liquid chromatography
- cell cycle arrest
- gas chromatography
- ms ms
- high resolution
- endoplasmic reticulum stress
- cell therapy
- cell death
- liquid chromatography tandem mass spectrometry
- oxidative stress
- signaling pathway
- stem cells
- tandem mass spectrometry
- mesenchymal stem cells
- solid phase extraction
- sensitive detection
- quantum dots
- simultaneous determination
- loop mediated isothermal amplification