Silica/Polyethylene Glycol Hybrid Materials Prepared by a Sol-Gel Method and Containing Chlorogenic Acid.
Michelina CatauroElisabetta TranquilloAlessia SalzilloLucia CapassoMichela IllianoLuigi SapioSilvio NaviglioPublished in: Molecules (Basel, Switzerland) (2018)
Chlorogenic acid (CGA) is a very common dietary polyphenolic compound. CGA is becoming very attractive due to its potential use as preventive and therapeutic agent in many diseases, including cancer. Inorganic/organic hybrid materials are gaining considerable attention in the biomedical field. The sol-gel process provides a useful way to obtain functional organic/inorganic hybrids. The aim of this study was to synthesize silica/polyethylene glycol (PEG) hybrids with different percentages of CGA by sol-gel technique and to investigate their impact on the cancer cell proliferation. Synthesized materials have been chemically characterized through the FTIR spectroscopy and their bioactivity evaluated looking by SEM at their ability to produce a hydroxyapatite layer on their surface upon incubation with simulated body fluid (SBF). Finally, their effects on cell proliferation were studied in cell lines by direct cell number counting, MTT, flow cytometry-based cell-cycle and cell death assays, and immunoblotting experiments. Notably, we found that SiO₂/PEG/CGA hybrids exhibit clear antiproliferative effects in different tumor, including breast cancer and osteosarcoma, cell lines in a CGA dependent manner, but not in normal cells. Overall, our results increase the evidence of CGA as a possible anticancer agent and illustrate the potential for clinical applications of sol-gel synthesized SiO₂/PEG/CGA materials.
Keyphrases
- cell cycle
- cell proliferation
- cell death
- flow cytometry
- papillary thyroid
- drug delivery
- cell cycle arrest
- induced apoptosis
- wound healing
- water soluble
- hyaluronic acid
- squamous cell
- working memory
- pi k akt
- signaling pathway
- childhood cancer
- endoplasmic reticulum stress
- cell therapy
- lymph node metastasis
- high throughput
- bone marrow
- oxidative stress
- single molecule
- climate change