3D high- and super-resolution imaging using single-objective SPIM.
Remi GallandGianluca GrenciAjay AravindVirgile ViasnoffVincent StuderJean-Baptiste SibaritaPublished in: Nature methods (2015)
Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations and features high background rejection and efficient photon collection, allowing for 3D single-molecule-based super-resolution imaging of whole cells or cell aggregates. Using larger mirrors enabled us to broaden the capabilities of our system to image Drosophila embryos.
Keyphrases
- single molecule
- high resolution
- living cells
- induced apoptosis
- high speed
- single cell
- cell cycle arrest
- cell therapy
- high throughput
- stem cells
- label free
- cell death
- machine learning
- optical coherence tomography
- loop mediated isothermal amplification
- bone marrow
- signaling pathway
- mesenchymal stem cells
- quantum dots
- monte carlo
- fluorescent probe
- sensitive detection