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A method to test antibody cross-reactivity toward animal antigens for flow cytometry.

Fulvio RiondatoBarbara ColittiSergio RosatiFederica SiniValeria Martini
Published in: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2022)
The availability of cross-reacting antibodies and/or of antibodies working in flow cytometry is a major issue in the veterinary field. One of the main problems is the availability of certain positive controls. With this brief communication, we report an method to quickly screen a wide number of products without the need to look for positive biological samples. We propose this approach as a first step to select the best antibodies to test on biological specimens.
Keyphrases
  • flow cytometry
  • mental health
  • dendritic cells
  • high throughput