NOAH-( 15 N/ 13 C)-CEST NMR supersequence for dynamics studies of biomolecules.

Rodrigo Cabrera Allpas Alexandar L Hansen Rafael Brüschweiler

Published in: Chemical communications (Cambridge, England) (2022)

An NMR supersequence is introduced for the rapid acquisition of 15 N-CEST and methyl- 13 C-CEST experiments in the same pulse sequence for applications to proteins. The high sensitivity and accuracy allows the simultaneous quantitative characterization of backbone and side-chain dynamics on the millisecond timescale ideal for routine screening for alternative protein states.

Keyphrases

high resolution
magnetic resonance
solid state
blood pressure
amino acid
protein protein
mass spectrometry
case control
small molecule