Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells.
Parivar YazdaniElham MansouriShirin EyvaziVahid YousefiHoman KahrobaMohammad Saeid HejaziAsghar MesbahiVahideh TarhrizMir Mahdi AbolghasemiPublished in: Artificial cells, nanomedicine, and biotechnology (2019)
Gene and drug delivery systems need crucial update in the issue of nanocarriers. Layered double hydroxides (LDHs) are known as biocompatible inorganic lamellar nanomaterials with versatile properties. In the present study, Zn/Al-LDH nanoparticle was synthesized and characterized by FTIR, XRD, SEM, TEM and Zeta potential tests and then intercalated with valproate and methyldopa by co-precipitation and ion exchange methods. These nanocarriers were applied as high activity nanolayers-based delivery systems. On the other hand, Zn/Al-LDH + plasmid/gene (pCEP4/Cdk9) evaluated on C2C12 myoblast cells. Co-operation loading indicated high efficiency of sorting and release of drugs. Additionally, the Real-Time PCR and Western blotting results for plasmid-gene (pCEP4/Cdk9) delivery showed that Zn/Al-LDH nanoparticles can be used as an effective carrier in cellular uptake and release of genes for gene therapy. Easy and cost-effective production of Zn/Al-LDH nanoparticles proposed them as potential alternatives for the traditional routs of drug/gene delivery.
Keyphrases
- genome wide
- genome wide identification
- escherichia coli
- copy number
- induced apoptosis
- heavy metals
- gene therapy
- high efficiency
- cell cycle arrest
- drug delivery
- crispr cas
- cell cycle
- real time pcr
- genome wide analysis
- transcription factor
- gold nanoparticles
- cell death
- emergency department
- endoplasmic reticulum stress
- ionic liquid
- climate change
- reduced graphene oxide
- adverse drug