Direct capture and selective elution of a secreted polyglutamate-tagged nanobody using bare magnetic nanoparticles.
Alexander A ZankerPatrick StargardtSophie C KurzbachChiara TurrinaJuergen MairhoferSebastian Patrick SchwamingerSonja BerensmeierPublished in: Biotechnology journal (2022)
Our studies clearly show that the unique combination of a growth-decoupled E. coli secretion system, the polyglutamate affinity tag, non-functionalized magnetic nanoparticles, and affinity magnetic precipitation is an innovative and novel way to capture and concentrate nanobodies.