Enhanced 13C NMR detects extended reaction networks in living cells.

Pernille Rose JensenFrancesca SannelliLudvig Tving StauningSebastian Meier

Published in: Chemical communications (Cambridge, England) (2021)

Insights into intracellular chemistry have remained sparse, but would be impactful for the advancement of biomedicine and bioproduction. A suitable 13C NMR approach provides improvements in sensitivity that make extended reaction networks and assay time windows, previously inaccessible cell densities and relative flux measurements accessible in living cells.

Keyphrases

living cells
fluorescent probe
magnetic resonance
high resolution
solid state
single molecule
single cell
cell therapy
reactive oxygen species
electron transfer
drug discovery
mesenchymal stem cells
bone marrow
mass spectrometry
neural network