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Targeted Gene Inactivations Expose Silent Cytochalasans in Magnaporthe grisea NI980.

Chongqing WangVerena HantkeRussell J CoxElizabeth J Skellam
Published in: Organic letters (2019)
The biosynthetic gene cluster encoding the phytotoxin pyrichalasin H 5 was discovered in Magnaporthe grisea NI980, and the late-stage biosynthetic pathway of 5 was fully elucidated using targeted gene inactivations resulting in the isolation of 13 novel cytochalasans. This study reveals that the nonproteinogenic amino acid O-methyltyrosine is the true precursor of 5, and other cryptic cytochalasans and mutasynthesis experiments produce novel halogenated pyrichalasin H analogues.
Keyphrases
  • copy number
  • genome wide
  • amino acid
  • genome wide identification
  • cancer therapy
  • gene expression
  • dna methylation