Functional Ex Vivo Assay Reveals Homologous Recombination Deficiency in Breast Cancer Beyond BRCA Gene Defects.

Titia G MeijerNicole S VerkaikAnieta M Sieuwerts Job van RietKishan A T NaipalCarolien H M van DeurzenMichael A den BakkerHein F B M SleddensHendrikus-Jan DubbinkT Dorine den ToomWinand N M DinjensEsther LipsPetra M NederlofMarcel SmidHarmen J G van de WerkenRoland KanaarJohn W M MartensAgnes Jager Dik C van Gent

Published in: Clinical cancer research : an official journal of the American Association for Cancer Research (2018)

RECAP is a robust functional HR assay detecting both BRCA1/2-deficient and BRCA1/2-proficient HRD tumors. Functional assessment of HR in a pseudo-diagnostic setting is achievable and produces robust and interpretable results.

Keyphrases

breast cancer risk
dna damage
high throughput
dna repair
genome wide
copy number
gene expression
young adults
transcription factor
single cell
genome wide identification