A Crosstalk- and Interferent-Free Dual Electrode Amperometric Biosensor for the Simultaneous Determination of Choline and Phosphocholine.
Rosanna CirielloAntonio GuerrieriPublished in: Sensors (Basel, Switzerland) (2021)
Choline (Ch) and phosphocholine (PCh) levels in tissues are associated to tissue growth and so to carcinogenesis. Till now, only highly sophisticated and expensive techniques like those based on NMR spectroscopy or GC/LC- high resolution mass spectrometry permitted Ch and PCh analysis but very few of them were capable of a simultaneous determination of these analytes. Thus, a never reported before amperometric biosensor for PCh analysis based on choline oxidase and alkaline phosphatase co-immobilized onto a Pt electrode by co-crosslinking has been developed. Coupling the developed biosensor with a parallel sensor but specific to Ch, a crosstalk-free dual electrode biosensor was also developed, permitting the simultaneous determination of Ch and PCh in flow injection analysis. This novel sensing device performed remarkably in terms of sensitivity, linear range, and limit of detection so to exceed in most cases the more complex analytical instrumentations. Further, electrode modification by overoxidized polypyrrole permitted the development of a fouling- and interferent-free dual electrode biosensor which appeared promising for the simultaneous determination of Ch and PCh in a real sample.
Keyphrases
- simultaneous determination
- liquid chromatography
- liquid chromatography tandem mass spectrometry
- tandem mass spectrometry
- ultra high performance liquid chromatography
- high resolution mass spectrometry
- high performance liquid chromatography
- label free
- room temperature
- solid phase extraction
- gold nanoparticles
- sensitive detection
- mass spectrometry
- quantum dots
- gas chromatography
- gene expression
- reduced graphene oxide
- nitric oxide
- solid state
- high resolution
- hydrogen peroxide
- ionic liquid